Purification and characterization of two tyrosyl-tRNA synthetase activities from soybean cotyledons

@inproceedings{Locy1978PurificationAC,
  title={Purification and characterization of two tyrosyl-tRNA synthetase activities from soybean cotyledons},
  author={Robert D. Locy and Joe H. Cherry},
  year={1978}
}
Abstract The tyrosyl- t RNA synthetases located in cytoplasm and chloroplasts of soybean cotyledons were purified to near homogeneity by ammonium sulfate precipitation, DEAE-cellulose chromatography, hydroxylapatite chromatography, and DEAE-Sephadex A-25 chromatography. Purified cytoplasmic tyrosyl- t RNA synthetase shows only a single band in acrylamide gel electrophoresis which corresponds to a MW of 126000. In SDS-acrylamide gel electrophoresis the enzyme again shows only a single band which… CONTINUE READING