Purification and characterization of two forms of endo-beta-1,4-mannanase from a thermotolerant fungus, Aspergillus fumigatus IMI 385708 (formerly Thermomyces lanuginosus IMI 158749).

@article{Puchart2004PurificationAC,
  title={Purification and characterization of two forms of endo-beta-1,4-mannanase from a thermotolerant fungus, Aspergillus fumigatus IMI 385708 (formerly Thermomyces lanuginosus IMI 158749).},
  author={Vladim{\'i}r Puchart and M{\'a}ria Vr{\vs}ansk{\'a} and Pavel Svoboda and J. Pohl and Z{\"u}mr{\"u}t B Ogel and Peter Biely},
  journal={Biochimica et biophysica acta},
  year={2004},
  volume={1674 3},
  pages={239-50}
}
Two extracellular endo-beta-1,4-mannanases, MAN I (major form) and MAN II (minor form), were purified to electrophoretic homogeneity from a locust bean gum-spent culture fluid of Aspergillus fumigatus IMI 385708 (formerly Thermomyces lanuginosus IMI 158749). Molecular weights of MAN I and MAN II estimated by SDS-PAGE were 60 and 63 kDa, respectively. IEF afforded several glycoprotein bands with pI values in the range of 4.9-5.2 for MAN I and 4.75-4.9 for MAN II, each exhibiting enzyme activity… CONTINUE READING

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