Purification and characterization of two endo-beta-1,4-glucanases from mollusca, Ampullaria crossean.

Abstract

Two novel endo-beta-1,4-glucanases, EG45 and EG27, were isolated from the gastric juice of mollusca, Ampullaria crossean, by anion exchange, hydrophobic interaction, gel filtration and a second round of anion exchange chromatography. The purified proteins EG45 and EG27 appeared as a single band on sodium dodecylsulfate polyacrylamide gel electrophoresis… (More)

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@article{Li2005PurificationAC, title={Purification and characterization of two endo-beta-1,4-glucanases from mollusca, Ampullaria crossean.}, author={Yanhong Li and Rui Guo and Qiu-yu Yin and Ming Ding and Siliang Zhang and Gen-jun Xu and Fu-kun Zhao}, journal={Acta biochimica et biophysica Sinica}, year={2005}, volume={37 10}, pages={702-8} }