Purification and characterization of two distinct acidic phytases with broad pH stability from Aspergillus niger NCIM 563

@inproceedings{Soni2010PurificationAC,
  title={Purification and characterization of two distinct acidic phytases with broad pH stability from Aspergillus niger NCIM 563},
  author={S. K. Soni and Amol Magdum and Jayant Malhar Khire},
  booktitle={World journal of microbiology & biotechnology},
  year={2010}
}
Aspergillus niger NCIM 563 produced two different extracellular phytases (Phy I and Phy II) under submerged fermentation conditions at 30°C in medium containing dextrin-glucose-sodium nitrate-salts. Both the enzymes were purified to homogeneity using Rotavapor concentration, Phenyl-Sepharose column chromatography and Sephacryl S-200 gel filtration. The molecular mass of Phy I and II as determined by SDS-PAGE and gel filtration were 66, 264, 150 and 148 kDa respectively, indicating that Phy I… CONTINUE READING

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References

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Showing 1-10 of 48 references

Differences in the active site

  • K Sethumadhavan
  • 1988
Highly Influential
4 Excerpts

Production studies and catalytic properties of phytases ( myoinositolhexakisphosphate phosphohydrolases ) : an overview

  • P Vats, UC Banerjee
  • Enzyme Microb Technol
  • 2004
Highly Influential
4 Excerpts

Fabrication of improved mini scale eletrofocusing unit

  • S Chinnatambi, AM Bodhe, AH Lachke
  • Biotechnol Tech
  • 1994
Highly Influential
3 Excerpts

Estimation of the molecular weights of proteins by Sephadex gel filtration

  • P Andrews
  • Biochem J
  • 1964
Highly Influential
3 Excerpts

Production and partial characterization of two types of phytase from Aspergillus niger NCIM 563 under submerged fermentation conditions

  • SK Soni, JM Khire
  • World J Microbiol Biotech
  • 2007
Highly Influential
4 Excerpts

Catalytic characterization of phytase (myoinositolhexakisphosphate phosphohydrolase) from Aspergillus niger van Teighem: Glycosylation pattern, kinetics and molecular

  • P Vats, UC Banerjee
  • 2006
Highly Influential
2 Excerpts

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