Purification and characterization of two distinct lipases from Candida cylindracea.

@article{Rua1993PurificationAC,
  title={Purification and characterization of two distinct lipases from Candida cylindracea.},
  author={L A Rua and Teresa Diaz-Maurino and Victor Manuel Fernandez and Cristina Otero and Antonio Ballesteros},
  journal={Biochimica et biophysica acta},
  year={1993},
  volume={1156 2},
  pages={181-9}
}
We have purified and characterized two isoenzymes from a commercial lipase preparation of Candida cylindracea. The purification procedure includes ethanol precipitation and DEAE-Sephacel and Sephacryl HR 100 chromatographies. Lipase A and lipase B were purified 11-fold with a 5% and 21% recovery in activity, respectively. The enzymes have similar amino acid content, N-terminal sequence and molecular weight, but differ on neutral sugar content, hydrophobicity, presence of isoforms and stability… CONTINUE READING