Purification and characterization of trypsin from the intestine of hybrid tilapia (Oreochromis niloticusxO.aureus).

@article{Wang2010PurificationAC,
  title={Purification and characterization of trypsin from the intestine of hybrid tilapia (Oreochromis niloticusxO.aureus).},
  author={Qin Wang and Zhi-Xing Gao and Nong Zhang and Yan Shi and Xiao-Lan Xie and Qing-Xi Chen},
  journal={Journal of agricultural and food chemistry},
  year={2010},
  volume={58 1},
  pages={655-9}
}
Trypsin from the intestine of hybrid tilapia (Oreochromis niloticus x O.aureus) was purified by the following techniques: acetone precipitation, ammonium sulfate fractionation, Sephacryl S-200 gel filtration, and DEAE-sephacel ion exchange chromatography. The purified enzyme was determined to be homogeneous by polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate (SDS)-PAGE. The molecular weight was estimated as 22,000 Da. The optimum pH and temperature of the enzyme for the… CONTINUE READING

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