Purification and characterization of toxins A and B of Clostridium difficile.

  title={Purification and characterization of toxins A and B of Clostridium difficile.},
  author={Nancy M Sullivan and Shahaireen Pellett and T. D. Wilkins},
  journal={Infection and immunity},
  volume={35 3},
Toxin preparations were obtained by growing Clostridium difficile VPI strain 10463 in 2-liter brain heart infusion dialysis flasks at 37 degrees C for 3 days. The initial step of the purification scheme involved ultrafiltration through an XM-100 membrane filter. Two toxic activities, designated toxins A and B, were separated by ion-exchange chromatography on DEAE-NaCl gradients. Toxin A was purified to homogeneity by an acetic acid precipitation at pH 5.5. Other separation techniques, including… CONTINUE READING


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