Purification and characterization of thermostable direct hemolysin of Vibrio parahaemolyticus.

@article{Sakurai1973PurificationAC,
  title={Purification and characterization of thermostable direct hemolysin of Vibrio parahaemolyticus.},
  author={J. J. Sakurai and Akinobu Matsuzaki and Toshio Miwatani},
  journal={Infection and immunity},
  year={1973},
  volume={8 5},
  pages={775-80}
}
A thermostable direct hemolysin was purified from culture filtrates of Vibrio parahaemolyticus. The purified hemolysin gave one precipitation line with the antihemolysin antiserum on agar-gel diffusion test and a single band on polyacrylamide gel electrophoresis. The hemolysin was not inactivated by heating at 70 to 100 C for 10 min. The hemolytic activity… CONTINUE READING