Purification and characterization of the rat liver gamma-butyrobetaine hydroxylase

@article{Galland2004PurificationAC,
  title={Purification and characterization of the rat liver gamma-butyrobetaine hydroxylase},
  author={S. Galland and F. Le Borgne and D. Guyonnet and P. Clouet and J. Demarquoy},
  journal={Molecular and Cellular Biochemistry},
  year={2004},
  volume={178},
  pages={163-168}
}
  • S. Galland, F. Le Borgne, +2 authors J. Demarquoy
  • Published 2004
  • Chemistry, Medicine
  • Molecular and Cellular Biochemistry
  • The biosynthesis of carnitine from lysine and methionine involves five enzymatic reactions. γ-butyrobetaine hydroxylase (BBH; EC 1.14.11.1) is the last enzyme of this pathway. It catalyzes the reaction of hydroxylation of γ-butyrobetaine to carnitine. This enzyme had never been purified to homogeneity from rat tissue. This paper describes the purification and characterization of the rat liver BBH. This protein has been purified some 413 fold by ion exchange, affinity and gel-filtration… CONTINUE READING
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