Purification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway.

@article{Meyer1992PurificationAC,
  title={Purification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway.},
  author={Eckart Meyer and Nelson J. Leonard and B. Shyamasundara Bhat and Joanne Stubbe and Joseph A. M. Smith},
  journal={Biochemistry},
  year={1992},
  volume={31 21},
  pages={5022-32}
}
Aminoimidazole riobnucleotide carboxylase, the sixth step in the purine biosynthetic pathway, catalyzes the conversion of aminoimidazole ribonucleotide (AIR) to carboxyaminoimidazole ribonucleotide (CAIR). The gene products of the purE and purK genes (PurE and PurK, respectively) thought to be responsible for this activity have been overexpressed and the proteins purified to homogeneity. PurE separates from PurK in the first ammonium sulfate fractionation during the purification. No evidence… CONTINUE READING

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