Purification and characterization of the Lactobacillus acidophilus bacteriocin lactacin B

@article{Barefoot1984PurificationAC,
  title={Purification and characterization of the Lactobacillus acidophilus bacteriocin lactacin B},
  author={S. Barefoot and T. Klaenhammer},
  journal={Antimicrobial Agents and Chemotherapy},
  year={1984},
  volume={26},
  pages={328 - 334}
}
Parameters for production and purification of a bacteriocin produced by Lactobacillus acidophilus N2 are described. Production of lactacin B was pH dependent, with maximum activity detected in broth cultures maintained at pH 6. Lactacin B was purified by ion-exchange chromatography, ultrafiltration, and successive gel filtrations in the presence of 8 M urea and then 0.1% sodium dodecyl sulfate. The molecular weight of lactacin B was ca. 6,000 to 6,500, and the purified compound showed maximum… Expand
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