Purification and characterization of the Lactobacillus acidophilus bacteriocin lactacin B

  title={Purification and characterization of the Lactobacillus acidophilus bacteriocin lactacin B},
  author={S. Barefoot and T. Klaenhammer},
  journal={Antimicrobial Agents and Chemotherapy},
  pages={328 - 334}
Parameters for production and purification of a bacteriocin produced by Lactobacillus acidophilus N2 are described. Production of lactacin B was pH dependent, with maximum activity detected in broth cultures maintained at pH 6. Lactacin B was purified by ion-exchange chromatography, ultrafiltration, and successive gel filtrations in the presence of 8 M urea and then 0.1% sodium dodecyl sulfate. The molecular weight of lactacin B was ca. 6,000 to 6,500, and the purified compound showed maximum… Expand
Purification and Partial Characterization of Novel Bacteriocin L23 Produced by Lactobacillusfermentum L23
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Identification and purification of a protein that induces production of the Lactobacillus acidophilus bacteriocin lactacin B
Lactacin B is a heat-stable bacteriocin produced by Lactobacillus acidophilus N2 that is active against closely related lactobacilli, including Lactobacillus delbrueckii subsp. lactis (formerlyExpand
Purification and partial characterization of bacteriocin produced by Lactococcus lactis ssp. lactis LL171
Bacteriocin reported in this study has the potential applications property as food preservative agent as well as unique and novel than reported bacteriocins. Expand
Purification and characterisation of acidocin D20079, a bacteriocin produced by Lactobacillus acidophilus DSM 20079.
Acidocin D20079 has a narrow inhibitory spectrum restricted to the genus Lactobacillus which includes L. sakei NCDO 2714, an organism known to cause anaerobic spoilage of vacuum-packaged meat. Expand
Caseicin 80: purification and characterization of a new bacteriocin from Lactobacillus casei
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Production, purification and chemical properties of an antistaphylococcal agent produced by Staphylococcus epidermidis.
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Purification and Some Properties of Diplococcin from Streptococcus cremoris 346
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Characterization of Lactobacillus sp. strain 100-37 from the murine gastrointestinal tract: ecology, plasmid content, and antagonistic activity toward Clostridium ramosum H1
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Mode of action of the staphylococcin-like peptide Pep 5 and culture conditions effecting its activity.
  • H. Sahl, H. Brandis
  • Biology, Medicine
  • Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...
  • 1982
A stable L-phase variant of Staphylococcus aureus, but not of Streptococcus faecalis, was susceptible to Pep 5, indicating that the selectivity of Pep 5 is not due to the presence of a cell wall receptor. Expand