Purification and characterization of the first bacterial laccase in the rhizospheric bacterium Azospirillum lipoferum

@article{Diamantidis2000PurificationAC,
  title={Purification and characterization of the first bacterial laccase in the rhizospheric bacterium Azospirillum lipoferum},
  author={G. Diamantidis and A. Effosse and P. Potier and R. Bally},
  journal={Soil Biology \& Biochemistry},
  year={2000},
  volume={32},
  pages={919-927}
}
Abstract Azospirillum lipoferum is a soil bacterium known for its ability to colonize roots and to promote plant growth. Recently, a laccase-like polyphenol oxidase activity has been reported in a strain isolated from the rhizosphere of rice. Purification to apparent homogeneity of A. lipoferum laccase was achieved after cell disruption by sonication, protein solubilization by Triton X-100, acetone and protamine precipitation, hydroxyapatite column chromatography in the presence of urea (5 M… Expand

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