Purification and characterization of the Staphylococcus aureus bacillithiol transferase BstA.

  title={Purification and characterization of the Staphylococcus aureus bacillithiol transferase BstA.},
  author={Varahenage Ranmali Perera and Gerald L. Newton and Jonathan M Parnell and Elizabeth A. Komives and Kit Pogliano},
  journal={Biochimica et biophysica acta},
  volume={1840 9},
BACKGROUND Gram-positive bacteria in the phylum Firmicutes synthesize the low molecular weight thiol bacillithiol rather than glutathione or mycothiol. The bacillithiol transferase YfiT from Bacillus subtilis was identified as a new member of the recently discovered DinB/YfiT-like Superfamily. Based on structural similarity using the Superfamily program, we have determined 30 of 31 Staphylococcus aureus strains encode a single bacillithiol transferase from the DinB/YfiT-like Superfamily, while… CONTINUE READING
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