Purification and characterization of the bacteriophage T7 gene 2.5 protein. A single-stranded DNA-binding protein.

@article{Kim1992PurificationAC,
  title={Purification and characterization of the bacteriophage T7 gene 2.5 protein. A single-stranded DNA-binding protein.},
  author={Young Tae Kim and Stanley Tabor and Carl D. Bortner and Jack D. Griffith and Charles C. Richardson},
  journal={The Journal of biological chemistry},
  year={1992},
  volume={267 21},
  pages={15022-31}
}
Bacteriophage T7 gene 2.5 protein has been purified to homogeneity from cells overexpressing its gene. Native gene 2.5 protein consists of a dimer of two identical subunits of molecular weight 25,562. Gene 2.5 protein binds specifically to single-stranded DNA with a stoichiometry of approximately 7 nucleotides bound per monomer of gene 2.5 protein; binding… CONTINUE READING