Purification and characterization of the single-stranded DNA binding protein from Streptococcus pneumoniae.

@article{Steffen2001PurificationAC,
  title={Purification and characterization of the single-stranded DNA binding protein from Streptococcus pneumoniae.},
  author={Scott E. Steffen and Floyd R. Bryant},
  journal={Archives of biochemistry and biophysics},
  year={2001},
  volume={388 1},
  pages={165-70}
}
The Escherichia coli single-stranded DNA binding (SSB) protein is a non-sequence-specific DNA binding protein that functions as an accessory factor for the RecA protein-promoted three-strand exchange reaction. An open reading frame encoding a protein similar in size and sequence to the E. coli SSB protein has been identified in the Streptococcus pneumoniae genome. The open reading frame has been cloned, an overexpression system has been developed, and the protein has been purified to greater… CONTINUE READING

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