Purification and characterization of sulfide dehydrogenase from alkaliphilic chemolithoautotrophic sulfur‐oxidizing bacteria

  title={Purification and characterization of sulfide dehydrogenase from alkaliphilic chemolithoautotrophic sulfur‐oxidizing bacteria},
  author={Dimitry Y. Sorokin and Govardus A. H. de Jong and Lesley A Robertson and Gijsbrecht Kuenen},
  journal={FEBS Letters},
Menaquinone reduction by an HMT2-like sulfide dehydrogenase from Bacillus stearothermophilus.
The gene-encoding HMT2-like sulfide dehydrogenase from Bacillus stearothermophilus JCM2501 was amplified and expressed in Escherichia coli and the enzymatic features were examined and sulfide oxidation via MK in the cellular membrane of Gram-positive bacteria was certified.
Catalytic Properties of Flavocytochrome c Sulfide Dehydrogenase from Haloalkaliphilic Bacterium Thioalkalivibrio paradoxus
Flavocytochrome c sulfide dehydrogenase was shown to oxidize exclusively sulfide but not other reduced sulfur compounds, such as thiosulfate, sulfite, tetrathionate, and sulfur, and it also does not catalyze the reverse reaction of sulfur reduction to sulfide.
Physiology of alkaliphilic sulfur-oxidizing bacteria from soda lakes
Alkaliphilic SOB isolated from soda lakes proved to be highly specialized for the doubly extreme conditions of high salt concentration and high pH and can be usefully exploited in bioreactors with stable soda alkalinity and supplied with inorganic sulfur compounds for sulfur removal from waste streams.
Pathways of sulfide oxidation by haloalkaliphilic bacteria in limited-oxygen gas lift bioreactors.
It is proposed that the bacteria reduce NAD(+) without direct transfer of electrons to oxygen and that this is most likely the main route for oxidizing sulfide to elemental sulfur which is subsequently oxidized to sulfate in oxygen-limited bioreactors, and is called the limited oxygen route (LOR).
Thioalkalispira microaerophila gen. nov., sp. nov., a novel lithoautotrophic, sulfur-oxidizing bacterium from a soda lake.
Phylogenetic analyses of the 16S rDNA sequences of strain ALEN 1(T) and its closest relatives demonstrated that this strain formed a deep branch within the gamma-Proteobacteria, with no obvious association to any described cluster of species/genera.
Halophilic and Haloalkaliphilic Sulfur-Oxidizing Bacteria
Investigation of hypersaline inland chloride-sulfate lakes and hypersaline brines of marine origin with neutral pH uncovered a novel and diverse world of extremophilic SOB with properties previously unknown for chemolithoautotrophic bacteria.
Development of oxygen control strategy towards sulfide oxidase production showed that the most favorable condition for the production of sulfidizing bacteria was without dissolved oxygen control, and the most superior sulfide oxidation producer was known as strain SO02.


Purification and some properties of two principal enzymes of the thiosulphate-oxidizing multi-enzyme system from Thiobacillus A2
Summary: A soluble thiosulphate-oxidizing multi-enzyme system, precipitated from a crude cell extract of Thiobacillus A2 with ammonium sulphate, has been resolved into four essential components by
Products of sulphide oxidation in extracts of Thiobacillus concretivorus.
Enzymic sulphide oxidation by thiobacillus concretivorus.
Flavocytochrome c of Chromatium vinosum. Some enzymatic properties and subunit structure.
The function and the structural features of Chromatium vinosum cy tochrome c-552 have been investigated and attempts to reconstitute the original flavocytochrome c from the subunits have been unsuccessful.
Studies on plant amylases: The effect of starch concentration upon the velocity of hydrolysis by the amylase of germinated barley.
  • C. Hanes
  • Biology, Medicine
    The Biochemical journal
  • 1932
From data collected within a few minutes of the origination of the reaction on the mixing of enzyme and substrate "initial slopes"' are determined graphically and used as a measure of reaction-velocity.
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products. Four major