Purification and characterization of sinapine synthase from seeds of Brassica napus.

  title={Purification and characterization of sinapine synthase from seeds of Brassica napus.},
  author={T. Vogt and R. Aebershold and B. Ellis},
  journal={Archives of biochemistry and biophysics},
  volume={300 2},
1-O-Sinapoylglucose:choline sinapoyltransferase ("sinapine synthase") has been purified from immature seeds of Brassica napus by sequential hydroxylapatite absorption, ion-exchange chromatography, and gel filtration. The purified enzyme has an apparent molecular weight of 65 kDa on gel filtration and a subunit structure on sodium dodecyl sulfate-polyacrylamide gel electrophoresis of 28 kDa. Sinapine synthase has Km values in the high micromolar range for both substrates (1-O-sinapoylglucose and… Expand
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