Purification and characterization of recombinant spider silk expressed in Escherichia coli

@article{Arcidiacono1998PurificationAC,
  title={Purification and characterization of recombinant spider silk expressed in Escherichia coli},
  author={Steven Arcidiacono and Charlene M. Mello and Drora Kaplan and Stephen Cheley and Hagan Bayley},
  journal={Applied Microbiology and Biotechnology},
  year={1998},
  volume={49},
  pages={31-38}
}
Abstract A partial cDNA clone, from the 3′ end of the dragline silk gene was isolated from Nephila clavipes major ampullate glands. This clone contains a 1.7-kb insert, consisting of a repetitive coding region of 1.4-kb and a 0.3-kb nonrepetitive coding region; 1.5-kb of the 1.7-kb fragment was cloned into Escherichia coli and a␣43-kDa recombinant silk protein was expressed. Characterization of the purified protein by Western blot, amino acid composition analysis, and matrix-assisted laser… 

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TLDR
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TLDR
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TLDR
A unique silk protein secreted from the cylindrical silk glands of the spider Nephila clavata, whose transcripts are homologous to one another in two termini and repetitive units, is described.

Stably Express Spider Flagelliform Silk Protein in Bombyx Mori Cell Line by PiggyBac Transposon-Derived Vector

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...

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