Purification and characterization of rat kidney sphingosine kinase.

  title={Purification and characterization of rat kidney sphingosine kinase.},
  author={Ana Olivera and Takafumi Kohama and Zhude Tu and Sheldon Milstien and Sarah Spiegel},
  journal={The Journal of biological chemistry},
  volume={273 20},
Sphingosine kinase catalyzes the formation of the bioactive sphingolipid metabolite sphingosine 1-phosphate, which plays important roles in numerous physiological processes, including growth, survival, and motility. We have purified rat kidney sphingosine kinase 6 x 10(5)-fold to apparent homogeneity. The purification procedure involved ammonium sulfate precipitation followed by chromatography on an anion exchange column. Partially purified sphingosine kinase was found to be stabilized by the… CONTINUE READING


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