Purification and characterization of pullulanase from Lactococcus lactis.

Abstract

This paper describes a simple and efficient method of isolation of a plullulanase type I from amylolytic lactic acid bacteria (ALAB). Extracellular pullulanase type I was purified from a cell-free culture supernatant of Lactococcus lactis IBB 500 by using ammonium sulfate fractionation and dialysis (instead of ultrafiltration), and ion-exchange… (More)
DOI: 10.1080/10826068.2011.575316

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