Purification and characterization of pullulanase from Lactococcus lactis.

  title={Purification and characterization of pullulanase from Lactococcus lactis.},
  author={Adam D Waśko and Magdalena Polak-Berecka and Zdzisław Targoński},
  journal={Preparative biochemistry & biotechnology},
  volume={41 3},
This paper describes a simple and efficient method of isolation of a plullulanase type I from amylolytic lactic acid bacteria (ALAB). Extracellular pullulanase type I was purified from a cell-free culture supernatant of Lactococcus lactis IBB 500 by using ammonium sulfate fractionation and dialysis (instead of ultrafiltration), and ion-exchange chromatography with CM Sepharose FF followed by gel filtration chromatography with Sephadex G-150 as the final step. A final purification factor of 14… CONTINUE READING