Purification and characterization of protein kinase C from the nematode Caenorhabditis elegans.

@article{Sassa1992PurificationAC,
  title={Purification and characterization of protein kinase C from the nematode Caenorhabditis elegans.},
  author={T Sassa and Junko Miwa},
  journal={The Biochemical journal},
  year={1992},
  volume={282 ( Pt 1)},
  pages={219-23}
}
Protein kinase C (PKC) of Caenorhabditis elegans was identified by enzymatic activity and [3H]phorbol 12,13-dibutyrate binding after DEAE-Sephacel column chromatography of a crude cytosolic extract. Ca(2+)-dependent activation of nematode PKC was observed in the presence of phosphatidylserine. The enzyme was maximally activated by 1,2-dioleoylglycerol or phorbol 12-myristate 13-acetate in the presence of phosphatidylserine and Ca2+. Hydroxyapatite column chromatography showed only one peak of… CONTINUE READING