Purification and characterization of oxalate oxidase from wheat seedlings

@article{Hu2008PurificationAC,
  title={Purification and characterization of oxalate oxidase from wheat seedlings},
  author={Yihong Hu and Zhenfei Guo},
  journal={Acta Physiologiae Plantarum},
  year={2008},
  volume={31},
  pages={229-235}
}
Oxalate oxidase (OxO, EC 1.2.3.4.) was purified to homogeneity from wheat (Triticum aestivum) seedlings by sequential thermal treatment, ultrafiltration, Sephadex G-100 gel filtration and affinity chromatography with concanavalin A. The enzyme was purified 66.11-fold with a recovery of 21.97%. It showed a subunit molecular mass of 32.6 kDa on SDS-PAGE and a native molecular mass of 170 kDa on Sephadex G-150 filtration, suggesting that it is a pentamer. The wheat OxO had a maximum activity at pH… CONTINUE READING