Purification and characterization of oil-bodies (oleosomes) and oil-body boundary proteins (oleosins) from the developing cotyledons of sunflower (Helianthus annuus L.)

@article{Millichip1996PurificationAC,
  title={Purification and characterization of oil-bodies (oleosomes) and oil-body boundary proteins (oleosins) from the developing cotyledons of sunflower (Helianthus annuus L.)},
  author={Mark Millichip and Arthur Sydney Tatham and Frank Jackson and Gareth Griffiths and Peter R Shewry and Allan Keith Stobart},
  journal={The Biochemical journal},
  year={1996},
  volume={314 ( Pt 1)},
  pages={333-7}
}
Oil-bodies, from the immature cotyledons of sunflower (Helianthus annuus L.), were difficult to purify to homogeneity using conventional techniques. The major protein contaminants were albumin and globulin storage proteins. A protocol has been developed, therefore, based upon the stringent washing of the oil-body fraction in 9 M urea, which effectively removed almost all the contaminating protein as judged by SDS/PAGE. The urea-washed oil-bodies were enriched in two major proteins of M(r) 19000… CONTINUE READING

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