Purification and characterization of laccase secreted by Phellinus linteus MTCC-1175 and its role in the selective oxidation of aromatic methyl group

@article{Chaurasia2013PurificationAC,
  title={Purification and characterization of laccase secreted by Phellinus linteus MTCC-1175 and its role in the selective oxidation of aromatic methyl group},
  author={Pankaj Kumar Chaurasia and Anju K. Yadav and Rama Shanker Singh Yadav and Sudha Yadava},
  journal={Applied Biochemistry and Microbiology},
  year={2013},
  volume={49},
  pages={592-599}
}
A laccase from the culture filtrate of Phellinus linteus MTCC-1175 has been purified to homogeneity. The method involved concentration of the culture filtrate by ammonium sulphate precipitation and an anion exchange chromatography on DEAE-cellulose. The SDS-PAGE and native-PAGE gave single protein band indicating that the enzyme preparation was pure. The molecular mass of the enzyme determined from SDS-PAGE analysis was 70 kDa. Using 2.6-dimethoxyphenol, 2.2′[azino-bis-(3-ethylbonzthiazoline-6… CONTINUE READING
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References

Publications referenced by this paper.
Showing 1-10 of 25 references

Appl. Biochem. Biotechnol

  • R Sahay, R S S Yadav, S Yadava
  • Appl. Biochem. Biotechnol
  • 2012

J. Mol. Cat. B: Enzymatic

  • A Coniglio, C Galli, P Gentili
  • J. Mol. Cat. B: Enzymatic
  • 2008

Appl. Biochem. Microbiol

  • O V Morozova, G P Shumakovich, S V Shleev, Y I Yaropolov
  • Appl. Biochem. Microbiol
  • 2007

Chem. Pharm. Bull

  • A Mikolasch, T H J Niedermeyer, M Lalk, S Witt, S Seefeldt, E Hammer
  • Chem. Pharm. Bull
  • 2007

Biotechnol. Adv

  • S R Couto, J L T Harrera
  • Biotechnol. Adv
  • 2006

Enz. Microb. Technol

  • H Zouri – Mechichi, T Mechichi, Dhouib Sayadi, S Marrtinez, A T Martinez
  • Enz. Microb. Technol
  • 2006

FEMS Microbiol. Rev

  • P Baldrian
  • FEMS Microbiol. Rev
  • 2006

Febs J

  • P J Hoegger, S Kilaru, T Y Jomes, J R Thacker, U Kuees
  • FEBS J
  • 2006

J. Biol. Inorg. Chem

  • I Bento, M Armenia, P F Corrondo, Lindloy
  • J. Biol. Inorg. Chem
  • 2006

Ind. Biotechnol

  • F Xu
  • Ind. Biotechnol
  • 2005

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