Purification and characterization of keratin hydrolase in psoriatic epidermis: application of keratin-agarose plate and keratin-polyacrylamide enzymography methods.

Abstract

Keratin-agarose plate and keratin-polyacrylamide enzymography methods were developed to demonstrate proteolytic digestion of epidermal keratin. By applying these methods, keratin hydrolase was purified from Tris-buffered saline extract of psoriatic scales by 50% ammonium sulfate precipitation, passage through a lysine-Sepharose column, DEAE-Sepharose… (More)

Topics

Figures and Tables

Sorry, we couldn't extract any figures or tables for this paper.