Purification and characterization of initiation factor IFE 3 from rabbit reticulocytes ( protein synthesis / multiprotein complex / 40 S ribosomal binding / reductive alkylation )

@inproceedings{HERSHEY2003PurificationAC,
  title={Purification and characterization of initiation factor IFE 3 from rabbit reticulocytes ( protein synthesis / multiprotein complex / 40 S ribosomal binding / reductive alkylation )},
  author={ROB BENNE AND JOHN W. B. HERSHEY},
  year={2003}
}
  • ROB BENNE AND JOHN W. B. HERSHEY
  • Published 2003
Initiation factor IF-E3 from rabbit reticulocytes was isolated from a high salt extract of ribosomes prepared according to the procedure of Schreier and Staehelin U. Mol. BioL 73, 329-349, 1973). The factor was highly purified from the crude extract by ammonium sulfate fractionation, sucrose zradietit centrifugation, salt gradient elution from DEAE-cellu… CONTINUE READING