Purification and characterization of hydrolytic haloalkane dehalogenase from Xanthobacter autotrophicus GJ10

@article{Keuning1985PurificationAC,
  title={Purification and characterization of hydrolytic haloalkane dehalogenase from Xanthobacter autotrophicus GJ10},
  author={S. Keuning and D. Janssen and B. Witholt},
  journal={Journal of Bacteriology},
  year={1985},
  volume={163},
  pages={635 - 639}
}
A new enzyme, haloalkane dehalogenase, was isolated from the 1,2-dichloroethane-utilizing bacterium Xanthobacter autotrophicus GJ10. The purified enzyme catalyzed the hydrolytic dehalogenation of n-halogenated C1 to C4 alkanes, including chlorinated, brominated, and iodinated compounds. The highest activity was found with 1,2-dichloroethane, 1,3-dichloropropane, and 1,2-dibromoethane. The enzyme followed Michaelis-Menten kinetics, and the Km for 1,2-dichloroethane was 1.1 mM. Maximum activity… Expand
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TLDR
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TLDR
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