Purification and characterization of human topoisomerase I mutants.


A system for rapid purification and characterization of eukaryotic topoisomerase-I mutants has been developed. The system utilizes six-histidine tagging of human topoisomerase I expressed in Saccharomyces cerevisiae to enable purification by nickel-affinity chromatography. Virtually homogenous mutant proteins are then tested for their ability to relax… (More)


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@article{Jensen1996PurificationAC, title={Purification and characterization of human topoisomerase I mutants.}, author={A. D. Jensen and Jesper Q. Svejstrup}, journal={European journal of biochemistry}, year={1996}, volume={236 2}, pages={389-94} }