Corpus ID: 20802250

Purification and characterization of human kidney cytosolic cysteine conjugate beta-lyase activity.

@article{Lash1990PurificationAC,
  title={Purification and characterization of human kidney cytosolic cysteine conjugate beta-lyase activity.},
  author={Lawrence H. Lash and Rita M. Nelson and R A Van dyke and M W Anders},
  journal={Drug metabolism and disposition: the biological fate of chemicals},
  year={1990},
  volume={18 1},
  pages={
          50-4
        }
}
  • L. Lash, R. Nelson, +1 author M. W. Anders
  • Published 1990
  • Chemistry, Medicine
  • Drug metabolism and disposition: the biological fate of chemicals
The central role of cysteine conjugate beta-lyase (beta-lyase) in the bioactivation of nephrotoxic halogenated hydrocarbons and the possibility of human exposure to these chemicals has focused interest on the beta-lyase from human kidney. Human kidney tissue was collected as surgical waste material, and subcellular fractions were isolated by differential centrifugation. Human beta-lyase activity, determined with S-(2-benzothiazolyl)-L-cysteine (BTC) as the substrate, was present in the… Expand
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