Purification and characterization of ethanol-inducible human hepatic cytochrome P-450HLj.

@article{Wrighton1987PurificationAC,
  title={Purification and characterization of ethanol-inducible human hepatic cytochrome P-450HLj.},
  author={Steven A. Wrighton and Paul E. Thomas and Dene E. Ryan and Wayne Levin},
  journal={Archives of biochemistry and biophysics},
  year={1987},
  volume={258 1},
  pages={292-7}
}
Human hepatic cytochrome P-450HLj was purified in a catalytically active state from microsomes obtained from an ethanol-intoxicated man. The electrophoretically homogeneous preparation of HLj was compared to rat P-450j and found to have a slightly different apparent molecular mass (54 vs 51.5 kDa) but highly similar immunochemical, spectral, and catalytic properties. Purified HLj exhibited high activity toward N-nitro-sodimethylamine (NDMA) and aniline metabolism, low but measurable activity… CONTINUE READING

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