Purification and characterization of human ZAP-70 protein-tyrosine kinase from a baculovirus expression system.

@article{Isakov1996PurificationAC,
  title={Purification and characterization of human ZAP-70 protein-tyrosine kinase from a baculovirus expression system.},
  author={N. Yu. Isakov and Ronald L. Wange and Julian D. Watts and Reudi Aebersold and Lawrence E Samelson},
  journal={The Journal of biological chemistry},
  year={1996},
  volume={271 26},
  pages={
          15753-61
        }
}
The ZAP-70 protein tyrosine kinase is essential for T cell antigen receptor (TCR)-mediated signaling. The absence of ZAP-70 results in impaired differentiation of T cells and a lack of responsiveness to antigenic stimulation. In order to study the characteristics of ZAP-70 in vitro, we overexpressed an epitopically tagged human ZAP-70 in a recombinant baculovirus expression system and purified it by column chromatography. The kinase activity of purified, recombinant ZAP-70 required cation and… CONTINUE READING

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