Purification and characterization of glyoxylate synthetase from greening potato-tuber chloroplasts.

  title={Purification and characterization of glyoxylate synthetase from greening potato-tuber chloroplasts.},
  author={Machhindra T. Janave and Nemmara Krishnan Ramaswamy and P. Madhusudanan Nair},
  journal={European journal of biochemistry},
  volume={214 3},
Glyoxylate synthetase catalyzing the condensation of two formate molecules into glyoxylate was purified to homogeneity by AcA-34, Sepharose CL-6B and DEAE-Sepharose CL-6B chromatography. A 150-fold purification with a specific activity of 25 mumol . mg protein-1 x 5 min-1 was obtained by this procedure. The reaction product was identified as glyoxylate. The enzyme was a tetramer having a molecular mass of 160 kDa with a subunit molecular mass of 40 kDa. The enzyme could be activated 3-4-fold by… 

A survey of carbon fixation pathways through a quantitative lens.

The main focus in this review is on thermodynamic and kinetic aspects, including thermodynamically challenging reactions, the ATP requirement of each pathway, energetic constraints on carbon fixation, and factors that are expected to limit the rate of the pathways.

C1 metabolism plays an important role during formaldehyde metabolism and detoxification in petunia under liquid HCHO stress.

Enhanced Formaldehyde Detoxification by Overexpression of Glutathione-Dependent Formaldehyde Dehydrogenase from Arabidopsis1

Results show that the capacity to take up and detoxify high concentrations of formaldehyde is proportionally related to the FALDH activity in the plant, revealing the essential role of this enzyme in formaldehyde detoxification.

Four components of the conjugated redox system in organisms: Carbon, nitrogen, sulfur, oxygen

A model of a three-component conjugated redox system is proposed in which the methyl group oxidation pathway is an archaic and conservative donor of protons and electrons, the reduction of O2 serves as an acceptor, and the arginine amino group is used for production of both urea and nitric oxide.

The Glycerate and Phosphorylated Pathways of Serine Synthesis in Plants: The Branches of Plant Glycolysis Linking Carbon and Nitrogen Metabolism

This review paper presents a hypothesis of the regulation of redox balance in stressed plant cells via participation of the reactions associated with glycerate and phosphorylated serine pathways, considered as important processes linking carbon and nitrogen metabolism and maintaining cellular redox and energy levels in stress conditions.



Partial purification and some properties of a latent CO2 reductase from green potato tuber chloroplasts.

The CO2 reductase, present in green potato tuber chloroplasts, as a latent form, was partially purified and showed 15-fold purification and 50% recovery of the activity, suggesting involvement of dithiol groups in the catalytic activity.

Isocitrate lyase from Pseudomonas indigofera. IV. Specificity and inhibition.

A novel pathway for the synthesis of solanidine in the isolated chloroplast from greening potatoes.

Isolated chloroplasts from green peelings were able to fix CO2 and the evidence obtained showed a direct reduction of CO2 to formate, which was the primary product of fixation.

o-Phthalaldehyde as a probe in the active site of phosphoenolpyruvate carboxylase.

Results indicate formation of two isoindoles showing the proximity of the essential lysine and cysteine residues at the active site of phosphoenolpyruvate carboxylase.