Purification and characterization of beta-glucosidase from honey bees (Apis mellifera).

  title={Purification and characterization of beta-glucosidase from honey bees (Apis mellifera).},
  author={J Pontoh and Nicholas H. Low},
  journal={Insect biochemistry and molecular biology},
  volume={32 6},
beta-glucosidase has been purified from the ventriculus and honey sac of Apis mellifera using a combination of anion- and cation-exchange, hydroxyapatite and gel-permeation chromatography. In addition, beta-glucosidase from the hypopharyngeal glands has been partially purified using anion-exchange and gel-permeation chromatography. The purified beta-glucosidase gave a positive result by glycoprotein staining. This beta-glucosidase consists of only one subunit and has M(r) of 72 kDa as… CONTINUE READING


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