Purification and characterization of cytosolic sialidase from rat liver.

  • T Miyagi, S Tsuiki
  • Published 1985 in The Journal of biological chemistry

Abstract

Sialidase has been purified from rat liver cytosol 83,000-fold by sequential chromatography on DEAE-cellulose, CM-cellulose, Blue-Sepharose, Sephadex G-200, and heparin-Sepharose. When subjected to sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis, the purified cytosolic sialidase moved as a single protein band with Mr = 43,000, a value similar… (More)

Cite this paper

@article{Miyagi1985PurificationAC, title={Purification and characterization of cytosolic sialidase from rat liver.}, author={T Miyagi and S Tsuiki}, journal={The Journal of biological chemistry}, year={1985}, volume={260 11}, pages={6710-6} }