Purification and characterization of chymotrypsin, trypsin and elastase like proteinases from cod (Gadus morhua L.).

Abstract

1. Chymotrypsin, trypsin and elastase have been purified from the pyloric caeca of cod. 2. The enzymes were separated by affinity/hydrophobic chromatography on phenyl-butyl-amine (PBA) substituted sepharose. Chymotrypsin eluted in two separate isozyme fractions whereas trypsin and elastase eluted in separate fractions consisting of two closely-related… (More)

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@article{Raae1989PurificationAC, title={Purification and characterization of chymotrypsin, trypsin and elastase like proteinases from cod (Gadus morhua L.).}, author={Arnt Johan Raae and Beverly Walther}, journal={Comparative biochemistry and physiology. B, Comparative biochemistry}, year={1989}, volume={93 2}, pages={317-24} }