Purification and characterization of chymotrypsin, trypsin and elastase like proteinases from cod (Gadus morhua L.).

@article{Raae1989PurificationAC,
  title={Purification and characterization of chymotrypsin, trypsin and elastase like proteinases from cod (Gadus morhua L.).},
  author={Arnt Johan Raae and Bernt Theodor Walther},
  journal={Comparative biochemistry and physiology. B, Comparative biochemistry},
  year={1989},
  volume={93 2},
  pages={
          317-24
        }
}
1. Chymotrypsin, trypsin and elastase have been purified from the pyloric caeca of cod. 2. The enzymes were separated by affinity/hydrophobic chromatography on phenyl-butyl-amine (PBA) substituted sepharose. Chymotrypsin eluted in two separate isozyme fractions whereas trypsin and elastase eluted in separate fractions consisting of two closely-related polypeptide chains as revealed by SDS-polyacrylamide electrophoresis and isoelectric focusing. 3. The cod enzymes consist of single polypeptide… CONTINUE READING
BETA

Citations

Publications citing this paper.
SHOWING 1-10 OF 10 CITATIONS

PURIFICATION OF CHYMOTRYPSIN FROM FISH WASTE USING REVERSE MICELLES

VIEW 4 EXCERPTS
CITES BACKGROUND & METHODS
HIGHLY INFLUENCED

Similar Papers

Loading similar papers…