Purification and characterization of cathepsin B from goat brain

Abstract

Cathepsin B was purified to an apparent homogeneity from goat brain utilizing the techniques of homogenization, autolysis at pH 4, 30–70% (NH4)2SO4 fractionation, Sephadex G-100 column chromatography, organomercurial afinity chromatography and ion-exchange chromatography on CM-Sephadex C-50. The enzyme had a pH optima of 6 with α-N-benzoyl-D, L-arginine… (More)
DOI: 10.1007/BF02702681

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@article{Kamboj2007PurificationAC, title={Purification and characterization of cathepsin B from goat brain}, author={Ramesh C. Kamboj and Suresh Pal and Hari Bansh Singh}, journal={Journal of Biosciences}, year={2007}, volume={15}, pages={397-408} }