Purification and characterization of brome mosaic virus RNA-dependent RNA polymerase.

@article{Quadt1990PurificationAC,
  title={Purification and characterization of brome mosaic virus RNA-dependent RNA polymerase.},
  author={Ralf Quadt and E. M. J. Jaspars},
  journal={Virology},
  year={1990},
  volume={178 1},
  pages={
          189-94
        }
}
RNA-dependent RNA polymerase (RdRp) was solubilized from cellular membranes of brome mosaic virus (BMV)-infected barley. The solubilized enzyme was subsequently purified by glycerol gradient centrifugation and DEAE ion-exchange chromatography. The purified enzyme proved to be highly stable and both dependent on and specific for BMV RNAs. The enzyme is inhibited by high template RNA concentrations. This inhibition indicates feedback regulation of minus-strand synthesis. The nonstructural viral… CONTINUE READING
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