Purification and characterization of basal apparatuses from a flagellate green alga.

@article{Geimer1997PurificationAC,
  title={Purification and characterization of basal apparatuses from a flagellate green alga.},
  author={Stefan Geimer and A Teltenk{\"o}tter and Uwe Plessmann and Klaus Weber and Karl F. Lechtreck},
  journal={Cell motility and the cytoskeleton},
  year={1997},
  volume={37 1},
  pages={
          72-85
        }
}
Basal apparatuses consisting of two basal bodies and several attached fibers were isolated from the naked green flagellate Spermatozopsis similis by detergent extraction and mechanical disintegration. Sucrose density centrifugation yielded highly enriched basal apparatuses as shown by electron microscopy. SDS-PAGE revealed the absence of histones, indicating the removal of nuclear contaminations from the isolated basal apparatuses. A mass spectrometric analysis of the carboxyterminal peptides… 
Isolation of the MLS-type Flagellar Apparatus from the an-1 mutant of Fern Lygodium japonicum and Its Preliminary Immunological Analysis
The basal apparatuses preserving the multilayered structure (MLS) were isolated from sperm of the an-1 strain of fern Lygodium japonicum by detergent extraction and DNase digestion, followed by
Distribution of polyglutamylated tubulin in the flagellar apparatus of green flagellates.
TLDR
Evidence is obtained that differences in the modification pattern can occur in a confined area of individual microtubules and the deficiency of polyglutamylated and detyrosinated tubulin in the transition region could have functional implications for flagellar turnover or excision.
A 210 kDa protein is located in a membrane-microtubule linker at the distal end of mature and nascent basal bodies.
A monoclonal antibody raised against purified flagellar basal apparatuses from the green flagellate Spermatozopsis similis reacted with a protein of 210 kDa (p210) in western blots. The protein was
Localization of p210-related proteins in green flagellates and analysis of flagellar assembly in the green alga Dunaliella bioculata with monoclonal anti-p210
TLDR
It is concluded that the pace and site by which individual proteins are integrated into the flagellum differ greatly.
Basal body replication in green algae--when and where does it start?
TLDR
Basal body duplication in the green alga Spermatozopsis similis was reinvestigated using GT335, an antibody binding to polyglutamylated tubulins, and antibodies directed to p210 to indicate that a new p210-containing structure forms near the proximal end of the basal bodies during basal body elongation, representing the precursor of the next generation of basal bodies.
Towards a Molecular Map of the Algal Basal Apparatus
TLDR
The progress in the biochemical identification and (immuno)localization of algal basal apparatus proteins is described starting with the discovery of the first alGal basal apparatus protein, centrin, to the most recent methodological advances involving highly purified basal apparatuses from the green alga Spermatozopsis similis (Geimer et al., 1997a).
A Novel 95-kD Protein Is Located in a Linker between Cytoplasmic Microtubules and Basal Bodies in a Green Flagellate and Forms Striated Filaments In Vitro
TLDR
Basal apparatus were isolated from the naked green flagellate Spermatozopsis similis and shown to be composed of several dozens of different polypeptides including a protein band of 95 kD, indicating that the 95-kD protein could be a major component of the sf-fibers, which interconnects specific triplets of the basal bodies with the microtubular bundles that emerge from the basal apparatus.
MITOSIS IN DUNALIELLA BIOCULATA (CHLOROPHYTA): CENTRIN BUT NOT BASAL BODIES ARE AT THE SPINDLE POLES
TLDR
It is demonstrated that centrin is present at the acentriolar spindle poles of Dunaliella independently from its localization in the basal apparatus.
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