Purification and characterization of bacteriocin from Pediococcus pentosaceus ACCEL.

  title={Purification and characterization of bacteriocin from Pediococcus pentosaceus ACCEL.},
  author={Chien-Wei Wu and Li-Jung Yin and Shann-Tzong Jiang},
  journal={Journal of agricultural and food chemistry},
  volume={52 5},
The Pediococcus pentosaceus ACCEL bacteriocin was purified to electrophoretical homogeneity by cell adsorption-desorption and Superose 12 fast performance liquid chromatography (FPLC). The purified bacteriocin, with a molecular mass of 17.5 kDa and an N-terminal sequence of -KYYGNGVTXGKHSXXVDXG-, belongs to class IIa and is designated pediocin ACCEL. It was inactivated by various proteases and stable at pH 2.0-6.0 and <100 degrees C. More than 80% activity was left even after 15 min of heating… CONTINUE READING
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