Purification and characterization of an extracellular protease produced by Pseudomonas fluorescens M3/6.

@article{Kohlmann1991PurificationAC,
  title={Purification and characterization of an extracellular protease produced by Pseudomonas fluorescens M3/6.},
  author={K L Kohlmann and S\oren Saxmose Nielsen and Michael R Ladisch},
  journal={Journal of dairy science},
  year={1991},
  volume={74 12},
  pages={4125-36}
}
Pseudomonas fluorescens strain M3/6 was inoculated into reconstituted NDM and incubated at 7 degrees C for 46 d. A significant amount of extracellular protease was produced, mainly during the latter part of the culture's life cycle. The protease was purified using ammonium sulfate fractionation, ion-exchange chromatography, and gel filtration. The isolated protease had activity on azocasein, alpha-, beta-, and kappa-caseins and a plasmin substrate but did not have plasminogen activator activity… CONTINUE READING