Purification and characterization of an outer membrane-bound protein involved in long-chain fatty acid transport in Escherichia coli.

@article{Black1987PurificationAC,
  title={Purification and characterization of an outer membrane-bound protein involved in long-chain fatty acid transport in Escherichia coli.},
  author={Paul N Black and Boctor Said and C R Ghosn and Jackie Beach and Walter Nunn},
  journal={The Journal of biological chemistry},
  year={1987},
  volume={262 3},
  pages={1412-9}
}
We report the purification and localization of the fadL gene product (FLP), an essential component of the long-chain fatty acid transport machinery in Escherichia coli. FLP was extracted from total membranes by differential extraction with the nonionic detergents Tween 20 and Triton X-100. This protein was further purified from a Tween 20-insoluble-Triton X-100-soluble extract by salt fractionation, gel filtration chromatography, and hydrophobic interaction chromatography. This regime results… CONTINUE READING
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