Purification and characterization of an extracellular beta-agarase from Bacillus sp. MK03.

  title={Purification and characterization of an extracellular beta-agarase from Bacillus sp. MK03.},
  author={Hisashi Suzuki and Yoshinori Sawai and Tohru S. Suzuki and Keiichi Kawai},
  journal={Journal of bioscience and bioengineering},
  volume={95 4},
A new beta-agarase was purified from an agarolytic bacterium, Bacillus sp. MK03. The enzyme was purified 129-fold from the culture supernatant by ammonium sulfate precipitation, anion exchange and gel filtration column chromatographic methods. The purified enzyme appeared as a single band on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Estimation of the molecular mass by SDS-PAGE and gel filtration gave values of 92 kDa and 113 kDa, respectively. The N-terminal amino… CONTINUE READING


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