Purification and characterization of an extracellular β-xylosidase from Pseudozyma hubeiensis NCIM 3574 (PhXyl), an unexplored yeast

  title={Purification and characterization of an extracellular $\beta$-xylosidase from Pseudozyma hubeiensis NCIM 3574 (PhXyl), an unexplored yeast},
  author={Nutan Mhetras and Susan Liddell and Digambar Vitthal Gokhale},
  journal={AMB Express},
This paper reports on the production of β-xylosidase from an unexplored yeast, Pseudozyma hubeinsis. The expression of this enzyme could be induced by beech wood xylan when the yeast was grown at 27 °C. The enzyme was purified to homogeneity as a glycoprotein with 23 % glycosylation. The purification protocol involved ammonium sulphate precipitation, QAE-Sephadex A50 ion exchange chromatography and sephacryl-200 column chromatography which resulted in 8.3-fold purification with 53.12 % final… 

Recovery and partial purification of thermophilic β-xylosidase derived from recombinant Bacillus megaterium MS941 by aqueous two-phase system

ABSTRACT A polymer–salt-based aqueous two-phase system (ATPS) was developed for the effective extraction and purification of extracellular β-xylosidase from the fermentation broth of recombinant

Production of Xylanase and β-Xylosidase Enzymes by Pseudozyma hubeiensis in Solid State Fermentation

The crude xylanase preparation of P. hubeiensis produced xylooligosaccharides (XOS) without xylose proving its potential for XOS production with no further requirement of downstream processing.

β-Xylosidases: Structural Diversity, Catalytic Mechanism, and Inhibition by Monosaccharides

The structural diversity and catalytic mechanisms of β-xylosidases are reviewed, and their inhibition by monosaccharides is discussed.

Development of a Vector Set for High or Inducible Gene Expression and Protein Secretion in the Yeast Genus Blastobotrys

Four new promising promoters, a new dominant marker, and two vectors for the secretion of epitope tagged proteins are reported along with a straightforward transformation protocol for direct and efficient conversion of renewable biomass.

Pseudozyma hubeiensis, an unexplored yeast: It’s potential in biomass conversion to value added products

The ability to grow at 40°C and no assimilation of erythritol are the characteristics that differentiate P. shanxiensis from all other species of Pseudozyma, and P. hubeiensis does not assimilate inositol and this characteristic differentiates P.hubeiensIS from all the other species.

New Insights into the Inhibition Mechanism of Betulinic Acid on α-Glucosidase.

Molecular docking showed that BA tightly bound to the active cavity of α-glucosidase, which might hinder the entrance of the substrate leading to a decline in enzyme activity.

Microbial cellulolytic enzymes: diversity and biotechnology with reference to lignocellulosic biomass degradation

This review article describes the structure and functions of divergent hydrolytic enzymes (cellulolytic and xylanolytic enzymes) involved in cellulosic biomass degradation, and analyzes, outlines and unveil the prospective source of microbes encoding exceptionally diverse set of biotechnologically relevant cellulolytic enzyme sources.