Purification and characterization of an extracellular β-xylosidase from Pseudozyma hubeiensis NCIM 3574 (PhXyl), an unexplored yeast

@article{Mhetras2016PurificationAC,
  title={Purification and characterization of an extracellular $\beta$-xylosidase from Pseudozyma hubeiensis NCIM 3574 (PhXyl), an unexplored yeast},
  author={Nutan Mhetras and Susan Liddell and Digambar Vitthal Gokhale},
  journal={AMB Express},
  year={2016},
  volume={6}
}
This paper reports on the production of β-xylosidase from an unexplored yeast, Pseudozyma hubeinsis. The expression of this enzyme could be induced by beech wood xylan when the yeast was grown at 27 °C. The enzyme was purified to homogeneity as a glycoprotein with 23 % glycosylation. The purification protocol involved ammonium sulphate precipitation, QAE-Sephadex A50 ion exchange chromatography and sephacryl-200 column chromatography which resulted in 8.3-fold purification with 53.12 % final… 

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