Purification and characterization of an efficient poultry feather degrading-protease from Myrothecium verrucaria

@article{MoreiraGasparin2009PurificationAC,
  title={Purification and characterization of an efficient poultry feather degrading-protease from Myrothecium verrucaria},
  author={Fabiana G. Moreira-Gasparin and Cristina Giatti Marques de Souza and A. Machado Costa and Ana Maria Alexandrino and Cissa K. Bracht and Cinthia Gandolfi Boer and Rosane Marina Peralta},
  journal={Biodegradation},
  year={2009},
  volume={20},
  pages={727-736}
}
The purpose of this work was to characterize an alkaline protease from the filamentous fungus Myrothecium verrucaria and to explore its capability to degrade native poultry feathers. The enzyme was purified to homogeneity using a single chromatographic step. Recovery was high, 62%, with a specific activity of 12,851.8 U/mg protein. The enzyme is a small monomeric protein with a molecular mass of 22 ± 1.5 kDa. It presented pH optimum of 8.3 and was stable over a broad pH range (5.0–12.0). The… CONTINUE READING

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