Purification and characterization of an alkaline lipase from Pseudomonas aeruginosa isolated from putrid mineral cutting oil as component of metalworking fluid.

@article{Karadi2006PurificationAC,
  title={Purification and characterization of an alkaline lipase from Pseudomonas aeruginosa isolated from putrid mineral cutting oil as component of metalworking fluid.},
  author={Ivanka Karad{\vz}i{\'c} and Akihiko Masui and Lidija Izrael Zivkovic and Nobuaki Fujiwara},
  journal={Journal of bioscience and bioengineering},
  year={2006},
  volume={102 2},
  pages={82-9}
}
Extracellular lipase was isolated and purified from the culture broth of Pseudomonas aeruginosa, an extremophile which naturally grows in water-soluble mineral cutting oil (pH 10) used as metalworking fluid (MWF) for cooling and lubrication in industrial metalworking processes. The molecular mass of the purified lipase was estimated by SDS-PAGE to be 54 kDa… CONTINUE READING