Purification and characterization of aminoglycoside 3'-phosphotransferase type IIa and kinetic comparison with a new mutant enzyme.

@article{Siregar1994PurificationAC,
  title={Purification and characterization of aminoglycoside 3'-phosphotransferase type IIa and kinetic comparison with a new mutant enzyme.},
  author={Johny Siregar and Stephen A. Lerner and Shahriar Mobashery},
  journal={Antimicrobial agents and chemotherapy},
  year={1994},
  volume={38 4},
  pages={
          641-7
        }
}
  • Johny Siregar, Stephen A. Lerner, Shahriar Mobashery
  • Published in
    Antimicrobial agents and…
    1994
  • Medicine, Chemistry
  • Aminoglycoside 3'-phosphotransferase [APH(3')s] provide an important means for high-level resistance to neomycin- and kanamycin-type aminoglycoside antibiotics. A four-step purification which affords milligram quantities of homogeneous APH(3') type IIa [APH(3')-IIa] is described. The kinetic parameters for the turnover of five substrates by the enzyme were determined, and the pH dependence and metal activation for catalysis were investigated. All five cysteines in the amino acid sequence of the… CONTINUE READING

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    Citations

    Publications citing this paper.
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    Bacterial resistance to aminoglycosides and beta-lactams: the Tn1331 transposon paradigm.

    • Marcelo E. Tolmasky
    • Medicine, Biology
    • Frontiers in bioscience : a journal and virtual library
    • 1999
    VIEW 1 EXCERPT
    CITES BACKGROUND

    Developing a snapshot of the ATP binding domain(s) of aminoglycoside phosphotransferases.

    VIEW 3 EXCERPTS
    CITES BACKGROUND

    References

    Publications referenced by this paper.
    SHOWING 1-10 OF 25 REFERENCES

    Synthesis of N - substituted , partially 0 - substituted derivatives of neamine and 2 - deoxystreptamine

    • P. N. Rather, R. S. Hare, G. H. Miller
    • Anal . Quim .
    • 1993

    Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins

    • A. Volbeda
    • J . Mol . Biol .
    • 1986