Purification and characterization of acyl carrier protein from two cyanobacteria species.

@article{Froehlich1990PurificationAC,
  title={Purification and characterization of acyl carrier protein from two cyanobacteria species.},
  author={John E. Froehlich and Roger A. Poorman and Edward Reardon and Susan R. Barnum and Jan G. Jaworski},
  journal={European journal of biochemistry},
  year={1990},
  volume={193 3},
  pages={817-25}
}
The acyl carrier protein (ACP), an essential protein cofactor for fatty acid synthesis, has been isolated from two cyanobacteria: the filamentous, heterocystous, Anabaena variabilis (ATCC 29211) and the unicellular Synechocystis 6803 (ATCC 27184). Both ACPs have been purified to homogeneity utilizing a three-column procedure. Synechocystis 6803 ACP was purified 1800-fold with 67% yield, while A. variabilis ACP was purified 1040-fold with 50% yield. Yields of 13.0 micrograms ACP/g Synechocystis… CONTINUE READING