Purification and characterization of a thermostable aliphatic amidase from the hyperthermophilic archaeon Pyrococcus yayanosii CH1

@article{Fu2014PurificationAC,
  title={Purification and characterization of a thermostable aliphatic amidase from the hyperthermophilic archaeon Pyrococcus yayanosii CH1},
  author={Ling Lin Fu and Xuegong Li and Xiang Xiao and Jun Xu},
  journal={Extremophiles},
  year={2014},
  volume={18},
  pages={429-440}
}
Amidases catalyze the hydrolysis of amides to free carboxylic acids and ammonia. Hyperthermophilic archaea are a natural reservoir of various types of thermostable enzymes. Here, we report the purification and characterization of an amidase from Pyrococcus yayanosii CH1, the first representative of a strict-piezophilic hyperthermophilic archaeon that… CONTINUE READING